PathoSense can receive yeast strains and aids with the successful isolation of long strands of DNA. Long read sequencing will help us to reconstruct complete chromosomes and to verify integration sites most effectively.
Know the strains you work with. Accelerate your biotech projects with precise yeast genome analysis.
Wasted months of development? Know where and what you implemented in the yeast genome by using long-read sequencing QC. Check for SNPs, plasmid integrations and structural variants.
Discover our yeast genomics solutions
DNA isolation & long-read sequencing
Draft or complete genome assembly
For many yeast strains used in biotech engineering, reference genomes are available. We can use reference-based genome assemblies to make a draft genome assembly of all chromosomes, including the mitochondrial chromosome. De novo genome assembly and polishing can be requested as well.
Variant analysis
Using long read assemble genomes we can identify the existence of structural variants in the chromosomes of the yeast. These can have influences in the phenotypical behaviour of the yeast strains used. Proper characterisation can aid in the selection of the most optimal clones for biotech experiments and protein production.
Plasmid integration sites
Plasmids are often used to plug in certain genes or gene cassettes into yeast chromosomes. Using the genomes generated with long reads, we can accurately identify the plasmid integration sites to ensure you work with correctly engineered yeast strains. Investing in sequencing for verification can save you a lot of time and money later on when you scale yeast production.